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Good paper on blackouts and dinos!
I found this fascinating paper on using blackouts to kill dinos! (Hey Google, why on earth didn't you kick this up in the gazillion previous searches I've done??) 3 day blackouts are *not* effective, it has to be at *least* 5 days, 8 for total kill.
A maximum of 75% of the cells were found to be dead—according to the Sytox staining technique—after 5 days in darkness. Duplicate sub-cultures from 3 day dark cultures resulted in regrowth of vegetative cells, after a short lag period. By contrast, duplicate sub-cultures from 8 day dark cultures produced no detectable growth (increases in fluorescence) over a three week monitoring period.
From: Mortality in cultures of the dinoflagellate Amphidinium carterae during culture senescence and darkness Daniel J. Franklin and John A. Berges (pdf file)
found during a search for photoperiod and cyanobacteria of all things.
Ivy
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28g cube, CF 105watts! Tunze 9001. Tiny frags: Euphyllia, blasto, ricordea and a rock flower anemone. Lost fish and inverts due to ongoing outbreak of dinoflagellates.
Current Tank Info: 28g aio, 105 watt CF lights, no sump or skimmer. 2 sexy shrimp, tiny frogspawn, tiny toadstool, tiny lps. Started Feb '15
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