Hanna calcium orange testrr

foneman02 · 02/11/2018, 07:57 PM

Just received Hanna calcium tester. Confused on procedure to test calcium level. Step one says to add1 ml of reagent A then add deionized water to 10 ml Mark . I assume this is Rodi water, to zero out meter. Then remove curvette from meter. First of all can’t they say vial. Then remove cap and add 0.1ml of sample. Do they mean tank water? Can’t they say tank water. Am I understanding this correctly

bertoni · 02/11/2018, 08:20 PM

As far as I understand this meter, the sample must be diluted to 9 parts RO/DI and 1 part tank water. Then you can 0.1 ml of your diluted sample, and put it into the meter. The dilution causes a loss of accuracy and precision, which is one of the downsides of this meter.

foneman02 · 02/12/2018, 04:05 AM

Thanks for getting back to me. Didn’t get chance to read your reply till this morning. Will attempt to run tests tonight after work and will compare against salifert test. Will post results.

bertoni · 02/12/2018, 11:15 AM

Okay, please let us know how well it works!

zachogden · 02/12/2018, 05:58 PM

Hanna has youtube videos for each of their meters that go through the test procedures step by step. I use them every time just to be sure.

josephxsxn · 02/13/2018, 06:26 AM

If you got the latest HANNA Calcium kit it should have a 1ml syringe with a plastic tip, a 150ul pipette with a plastic tip, and a larger maybe 5ml plastic dropperish thing (cant recall name.)

You would take 1ml of reagent A the liquid into the 1ml plastic tip (its volumetric) and place it into the vial. Then fill the vial the rest of the way with RO/DI water to the 10ml mark.. Place into colorimeter and when you see it says C1 press. Remove tube from the colorimeter and take the 150ul pipette with tip and place so that its about 4 -5 inches into the sample water and press down to the FIRST resistance barrier on the pipette, then release and wait 5 seconds. Remove the pipette with the sample and place it into the test tube your using by pressing all the way down past the first resistance barrier and to the second.. Now add Reagent B, shake well for the duration (dont recall) and then wait 15 seconds for the bubbles to clear up and test.

This is pretty much how I do mine every time.

+1 to the vid idea, ill have to check them out.

02/11/2018, 07:57 PM	#1
foneman02 Registered Member Join Date: Feb 2012 Posts: 22	Hanna calcium orange testrr Just received Hanna calcium tester. Confused on procedure to test calcium level. Step one says to add1 ml of reagent A then add deionized water to 10 ml Mark . I assume this is Rodi water, to zero out meter. Then remove curvette from meter. First of all can’t they say vial. Then remove cap and add 0.1ml of sample. Do they mean tank water? Can’t they say tank water. Am I understanding this correctly

02/11/2018, 08:20 PM	#2
bertoni RC Mod Join Date: Mar 2002 Location: Mountain View, CA, USA Posts: 88,616	As far as I understand this meter, the sample must be diluted to 9 parts RO/DI and 1 part tank water. Then you can 0.1 ml of your diluted sample, and put it into the meter. The dilution causes a loss of accuracy and precision, which is one of the downsides of this meter. __________________ Jonathan Bertoni

02/12/2018, 11:15 AM	#4
bertoni RC Mod Join Date: Mar 2002 Location: Mountain View, CA, USA Posts: 88,616	Okay, please let us know how well it works! __________________ Jonathan Bertoni

02/12/2018, 04:05 AM	#3
foneman02 Registered Member Join Date: Feb 2012 Posts: 22	Thanks for getting back to me. Didn’t get chance to read your reply till this morning. Will attempt to run tests tonight after work and will compare against salifert test. Will post results.

02/12/2018, 05:58 PM	#5
zachogden Registered Member Join Date: Sep 2013 Posts: 88	Hanna has youtube videos for each of their meters that go through the test procedures step by step. I use them every time just to be sure.

02/13/2018, 06:26 AM	#6
josephxsxn Registered Member Join Date: Oct 2017 Location: DTW, Michigan Posts: 122	If you got the latest HANNA Calcium kit it should have a 1ml syringe with a plastic tip, a 150ul pipette with a plastic tip, and a larger maybe 5ml plastic dropperish thing (cant recall name.) You would take 1ml of reagent A the liquid into the 1ml plastic tip (its volumetric) and place it into the vial. Then fill the vial the rest of the way with RO/DI water to the 10ml mark.. Place into colorimeter and when you see it says C1 press. Remove tube from the colorimeter and take the 150ul pipette with tip and place so that its about 4 -5 inches into the sample water and press down to the FIRST resistance barrier on the pipette, then release and wait 5 seconds. Remove the pipette with the sample and place it into the test tube your using by pressing all the way down past the first resistance barrier and to the second.. Now add Reagent B, shake well for the duration (dont recall) and then wait 15 seconds for the bubbles to clear up and test. This is pretty much how I do mine every time. +1 to the vid idea, ill have to check them out.