Reef Central Online Community

Go Back   Reef Central Online Community > General Interest Forums > New to the Hobby
Blogs FAQ Calendar Mark Forums Read

Notices

User Tag List

Reply
Thread Tools
Unread 01/03/2018, 10:28 AM   #1
aby
Registered Member
 
Join Date: Jan 2015
Posts: 31
How do You Culture Nannochloropsis?

Can someone please tell me how to culture nannochloropsis?

I don't have any idea, but after seeing below Youtube video:

https://www.youtube.com/watch?v=SaAFQlFC7fI

I think I understand how to do it.

All I need to do is buy a 1 Liter starter package of nannochloropsis, then divide it into 2 mineral water bottle (1 Liter each) and add this 2 Liter of nannochloropsis with saltwater until full. Then I need water pump into each of this 2 Liter to provide an oxygen. How long to wait until the nannochloropsis full grown?

Am I correct?


aby is offline   Reply With Quote
Unread 01/03/2018, 01:08 PM   #2
sde1500
Registered Member
 
sde1500's Avatar
 
Join Date: Mar 2015
Location: Glocester, RI
Posts: 3,336
It is pretty easy, and mostly what you said. If you buy a liquid sample then yes, split the sample into two bottles, fill the bottles with the sample and new salt water. Run some airline into each from an air pump to get it bubbling, add light. Also helps to fertilize, Gullard's f/2 is the way to go.

Most any white light will do, this was my first set up, culturing both Nanno and Tetraselmis.


Upgraded to make it a little neater:


I tend to split it weekly, you should see the bottles get nice and dark green. They do sell density measuring sticks to stick in the bottles and know how dense the culture is, but I've never bothered with it.


__________________
My build thread:

http://reefcentral.com/forums/showthread.php?t=2548422

Current Tank Info: 65 gallon mixed reef, Eshopps sump and HOB overflow, RO-110int skimmer, Reefbreeder 32" photons V1.
sde1500 is offline   Reply With Quote
Unread 01/03/2018, 01:15 PM   #3
sde1500
Registered Member
 
sde1500's Avatar
 
Join Date: Mar 2015
Location: Glocester, RI
Posts: 3,336
I should add, you are brand spanking new to this from your other posts. While culturing phyto is kinda easy, you really don't need it. I do it for fun, to feed some other things I culture. I'd highly suggest you do not start this, until you have your tank up and running and you understand the care going into the tank itself. Not to be too critical, but when you are posting trying to figure out what a corner overflow is called, you definitely don't have much need to be trying to learn to culture phytoplankton.


__________________
My build thread:

http://reefcentral.com/forums/showthread.php?t=2548422

Current Tank Info: 65 gallon mixed reef, Eshopps sump and HOB overflow, RO-110int skimmer, Reefbreeder 32" photons V1.
sde1500 is offline   Reply With Quote
Unread 01/03/2018, 01:37 PM   #4
aby
Registered Member
 
Join Date: Jan 2015
Posts: 31
So, is dark green a sign of growing nannochloropsis?

Also, I know nannochloropsis is a food for copepod but what tetraselmis for?


aby is offline   Reply With Quote
Unread 01/03/2018, 02:23 PM   #5
sde1500
Registered Member
 
sde1500's Avatar
 
Join Date: Mar 2015
Location: Glocester, RI
Posts: 3,336
Yes if the color darkens a lot, it is growing well. And actually in my experience, nanno being non-motile does not make a good food source for my pod cultures. I would use it and the water would stay green, indicating they weren't eating it, plus there is a slightly hard shell around the nanno cells that pods struggle with. Tetraselmis is another phytoplankton, motile and with less of a outer shell, making it a better food.


__________________
My build thread:

http://reefcentral.com/forums/showthread.php?t=2548422

Current Tank Info: 65 gallon mixed reef, Eshopps sump and HOB overflow, RO-110int skimmer, Reefbreeder 32" photons V1.
sde1500 is offline   Reply With Quote
Unread 01/04/2018, 11:52 AM   #6
aby
Registered Member
 
Join Date: Jan 2015
Posts: 31
Quote:
Originally Posted by sde1500 View Post
...Tetraselmis is another phytoplankton, motile and with less of a outer shell, making it a better food.
Better food for what?


aby is offline   Reply With Quote
Unread 01/04/2018, 11:55 AM   #7
sde1500
Registered Member
 
sde1500's Avatar
 
Join Date: Mar 2015
Location: Glocester, RI
Posts: 3,336
You mentioned pods, so pods.


__________________
My build thread:

http://reefcentral.com/forums/showthread.php?t=2548422

Current Tank Info: 65 gallon mixed reef, Eshopps sump and HOB overflow, RO-110int skimmer, Reefbreeder 32" photons V1.
sde1500 is offline   Reply With Quote
Unread 01/04/2018, 01:59 PM   #8
aby
Registered Member
 
Join Date: Jan 2015
Posts: 31
Are you sure Tetraselmis is a better food for copepods than nannochloropsis?

Why other site say and suggest nannochloropsis is most common food for copepods?


aby is offline   Reply With Quote
Unread 01/04/2018, 02:10 PM   #9
sde1500
Registered Member
 
sde1500's Avatar
 
Join Date: Mar 2015
Location: Glocester, RI
Posts: 3,336
Common and better have two wildly different meanings.

Why do you want to culture phyto and pods?


__________________
My build thread:

http://reefcentral.com/forums/showthread.php?t=2548422

Current Tank Info: 65 gallon mixed reef, Eshopps sump and HOB overflow, RO-110int skimmer, Reefbreeder 32" photons V1.
sde1500 is offline   Reply With Quote
Unread 01/04/2018, 02:15 PM   #10
aby
Registered Member
 
Join Date: Jan 2015
Posts: 31
Quote:
Originally Posted by sde1500 View Post
...Why do you want to culture phyto and pods?
I want to culture copepods for feeding my fish. To culture copepods, I need to culture phytoplankton too because it's a food for copepods.


aby is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is On



All times are GMT -6. The time now is 03:44 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Powered by Searchlight © 2024 Axivo Inc.
Use of this web site is subject to the terms and conditions described in the user agreement.
Reef CentralTM Reef Central, LLC. Copyright ©1999-2022
User Alert System provided by Advanced User Tagging v3.3.0 (Pro) - vBulletin Mods & Addons Copyright © 2024 DragonByte Technologies Ltd.