|
12/02/2011, 07:51 PM | #1 |
Team RC Member
Join Date: Aug 2008
Location: Highland, Maryland Entomologist
Posts: 14,591
|
Interesting article regarding current knowlege of anammox bacteria & the biochemistry
Critical Reviews in Biochemistry and Molecular Biology, 2009; 44(2–3): 65–84
Biochemistry and molecular biology of anammox bacteria http://informahealthcare.com/doi/pdf...09230902722783 Mike S. M. Jetten1,2, Laura van Niftrik1, Marc Strous1, Boran Kartal1, Jan T. Keltjens1, and Huub J. M. Op den Camp1 1Department of Microbiology, IWWR, Faculty of Science, Radboud University of Nijmegen, Toernooiveld 1, NL-6525 ED Nijmegen, The Netherlands, and 2Department of Biotechnology, Delft University of Technology, Julianalaan 67, NL-2628 BC Delft, The Netherlands Abstract (Enitre article is available to read) Anaerobic ammonium-oxidizing (anammox) bacteria are one of the latest additions to the biogeochemical nitrogen cycle. These bacteria derive their energy for growth from the conversion of ammonium and nitrite into dinitrogen gas in the complete absence of oxygen. These slowly growing microorganisms belong to the order Brocadiales and are affiliated to the Planctomycetes. Anammox bacteria are characterized by a compartmentalized cell architecture featuring a central cell compartment, the “anammoxosome”. Thus far unique “ladderane” lipid molecules have been identified as part of their membrane systems surrounding the different cellular compartments. Nitrogen formation seems to involve the intermediary formation of hydrazine, a very reactive and toxic compound. The genome of the anammox bacterium Kuenenia stuttgartiensis was assembled from a complex microbial community grown in a sequencing batch reactor (74% enriched in this bacterium) using a metagenomics approach. The assembled genome allowed the in silico reconstruction of the anammox metabolism and identification of genes most likely involved in the process. The present anammox pathway is the only one consistent with the available experimental data, thermodynamically and biochemically feasible, and consistent with Ockham’s razor: it invokes minimum biochemical novelty and requires the fewest number of biochemical reactions. The worldwide presence of anammox bacteria has now been established in many oxygen-limited marine and freshwater systems, including oceans, seas, estuaries, marshes, rivers and large lakes. In the marine environment over 50% of the N2 gas released may be produced by anammox bacteria. Application of the anammox process offers an attractive alternative to current wastewater treatment systems for the removal of ammonia-nitrogen. Currently, at least five full scale reactor systems are operational. ------------------------------------------------------------------------ Perhaps the use of anaerobic reactors with acetate as a carbon source may be a more effective way for reducing nitrate in a reef aquarium.
__________________
Cliff Babcock Intestests: Digital Microscopy; Marine Pest Control; Marine Plants & Macroalgae Current Tank Info: 180 g. mixed reef system |
12/02/2011, 08:43 PM | #2 |
Registered Member
Join Date: Feb 2007
Location: Canada
Posts: 212
|
So am I correct to interpret this as bringing credence to plenums and DSB's employed in our hobby?
__________________
Stu Retired 500g system... Researching and building a nice and relaxing 65g display |
12/03/2011, 07:18 AM | #3 |
Team RC Member
Join Date: Aug 2008
Location: Highland, Maryland Entomologist
Posts: 14,591
|
This review discusses the benefits of anaerobic reactors which can be used to either replace or act in series with aerobic reactors for reducing nutrients in waste water management, aquaculture & other applications. They are saying that anaerobic reactors are cheaper & reduce more CO2 out of the water than aerobic reactors, which would be beneficial to reef aquariums. They state the carbon sources used are important when using anaerobic reactors since alcohols (including ethanol) inhibit ANAMMOX bacteria. Acetate (vinegar) is one carbon source that works well with ANAMMOX bacteria.
I have not seen anaerobic bioreactors used in the hobby much with the exception of sulfur reactors which have their concerns. From threads I have read here at RC, there are questions regarding how effective deep sand beds are in a reef aquarium. ANAMMOX bacteria grow relatively slowly compared to aerobic bacteria without a carbon source, but when the scientists add acetate (vinegar) this greatly increases their effectiveness for reducing excess nutrients in the water column. Perhaps dosing vinegar in a reef aquarium with a deep sand bed will increase nitrate reduction to where it is a much more useful tool.
__________________
Cliff Babcock Intestests: Digital Microscopy; Marine Pest Control; Marine Plants & Macroalgae Current Tank Info: 180 g. mixed reef system |
12/03/2011, 04:11 PM | #4 |
Registered Member
Join Date: Feb 2007
Location: Canada
Posts: 212
|
I think Mr. Wilson is already doing this. I think I had read somewhere that he has an airline tube threaded down through a deep sand bed (in his overflow) and doses. I may be remembering this wrong, but I'll see if I can find it.
Very interesting.
__________________
Stu Retired 500g system... Researching and building a nice and relaxing 65g display |
12/03/2011, 04:12 PM | #5 |
-RT * ln(k)
Join Date: Sep 2010
Location: Little Rock
Posts: 9,705
|
Cool article Cliff.
__________________
David Current Tank: Undergoing reconstruction... |
12/03/2011, 04:37 PM | #6 | |
Registered Member
Join Date: Feb 2011
Location: Houston, TX
Posts: 270
|
Quote:
|
|
12/03/2011, 05:17 PM | #7 |
Team RC Member
Join Date: Aug 2008
Location: Highland, Maryland Entomologist
Posts: 14,591
|
Most data available regarding nutrient reduction comes from waste fresh water treatment studies. There have been a few studies completed involving seawater waste removal which seems to follow suit with the freshwater studies.
This article provides a good comparison of anaerobic vs aerobic bacterial nutrient break-down for fresh water: ANAEROBIC VERSUS AEROBIC TREATMENT IN THE U.S.A. http://www.google.com/url?sa=t&rct=j...8jzpBnoVaaDE3Q From the graphs in this article, anaerobic break-down is the winner as far as cost and provides the best nutrient break-down (significantly lower than aerobic alone and the combo of the two). The chemical pathways for reduction seem to be the most efficient IMHO using anaerobic methods. From the graphs the anaerobic bacteria provide the lowest final nutrient levels. The studies I have read indicate that ethanol will inhibit the anaerobic bacteria which is not a good thing IMHO. On the other hand acetate (vinegar) will increase growth rates of both anaerobic and aerobic bacteria which is a win, win IMHO. So to answer your question, IMHO vinegar alone may be the clear winner based on what info I have read. Vinegar is the cheapest carbon source as well, which makes it a winner as far as long term costs. The biopellets are just too expensive at this point and I see no need for their use. Vodka is more expensive than vinegar.
__________________
Cliff Babcock Intestests: Digital Microscopy; Marine Pest Control; Marine Plants & Macroalgae Current Tank Info: 180 g. mixed reef system Last edited by HighlandReefer; 12/03/2011 at 05:26 PM. |
12/03/2011, 05:41 PM | #8 |
Team RC Member
Join Date: Aug 2008
Location: Highland, Maryland Entomologist
Posts: 14,591
|
As far as reactors that hobbyists can use to take advantage of the anaerobic pathway, while using vinegar as a carbon source for nutrient reduction, perhaps Randy is currently using a very good method.
It is my understanding that Randy is currently using a canister filter with GAC in it and when he cleans it out he finds lots of bacterial growth in it, which he rinses as necessary to keep flow going. Randy does a lot of vinegar as well. A canister filter with reduced flow that produces lots of bacterial masses (biolfims) will produce a lot more anaerobic bacteria than a reactor with biopellets in it as hobbyists are currently using, unless they really slow down the flow and allow a lot of bacterial biomass to develop. Most hobbyists don't do this with the pellet reactors from the pictures I have seen. Perhaps this is a reason why many hobbyists seem to have problems with the pellet reactors? For anaerobic bacterial production it is all about the production of large bacterial biomasses which contain the anaerobic areas needed for the bacteria to grow.
__________________
Cliff Babcock Intestests: Digital Microscopy; Marine Pest Control; Marine Plants & Macroalgae Current Tank Info: 180 g. mixed reef system |
12/03/2011, 05:59 PM | #9 |
Team RC Member
Join Date: Aug 2008
Location: Highland, Maryland Entomologist
Posts: 14,591
|
Another advantage for using vinegar is that it will increase anaerobic break-down of nutrients within sand beds and the surface of rocks where cyano tends to grow if space is available which is a survival of the most fit situation. If enough anaerobic bacterial growth (biofilms) are produced by dosing vinegar in these sites than the anaerobic bacteria prevail and out-compete the cyano. On the other hand vodka will inhibit the anaerobic bacteria at these sights which may allow the cyano to growth instead which results in cyano out-competing the aerobic bacteria. Cyanobacteria utilize photosynthesis which aerobic bacteria can't in most cases, which gives the cyano a big edge to outcompete the aerobic bacteria. IMO, anaerobic bacteria are your best bet to out-compete the cyano. Therefore, acetate (vinegar) dosing Win-Win-Win-Win.
__________________
Cliff Babcock Intestests: Digital Microscopy; Marine Pest Control; Marine Plants & Macroalgae Current Tank Info: 180 g. mixed reef system Last edited by HighlandReefer; 12/03/2011 at 06:16 PM. |
12/03/2011, 06:11 PM | #10 | |
Registered Member
Join Date: Aug 2010
Posts: 74
|
Quote:
Sent from my GT-I9000 using Tapatalk |
|
12/03/2011, 06:26 PM | #11 |
Team RC Member
Join Date: Aug 2008
Location: Highland, Maryland Entomologist
Posts: 14,591
|
Most dinos are phototrophic like cyano and algae. Many dinos can consume algae, bacteria & other dinos as a food source depending on the specie involved, so limiting phosphate like for cyano does not work well for many dino species. So, again, IMO using anaerobic bacteria may be your best bet to try and out-compete dinos while using vinegar to increase the normal slow anaerobic bacterial growth.
Simply dosing vinegar will not be the total answer when trying to control dinos, you will still need to use the other means to reduce nutrients in a reef tank. If you have areas where rock and sand beds are covered with cyano, algae or dinos it will not be easy for the anaerobic bacteria to establish themselves in place of the pests since these pests all produce toxins to inhibit other bio growth. By removing the pests, you than allow the bacteria bioflims to develop and perhaps out-compete the pests.
__________________
Cliff Babcock Intestests: Digital Microscopy; Marine Pest Control; Marine Plants & Macroalgae Current Tank Info: 180 g. mixed reef system |
12/03/2011, 06:43 PM | #12 |
Team RC Member
Join Date: Aug 2008
Location: Highland, Maryland Entomologist
Posts: 14,591
|
Perhaps yet another advantage for using acetate (vinegar).
From this article two species of cyanobacterial (blue green algae) were tested using acetate and it did not increase the growth of these species. The Metabolism of Acetate by the Blue-green Algae, Anabaena variabilis and Anacystis nidulans http://www.google.com/url?sa=t&rct=j...DaujP-P9TbjPnA From it: RESULTS Growth arid respiratory studies Short-term experiments. When the minimal salt (autotrophic) medium was supplemented with sodium acetate (20 mM) neither Anabaena variabilis nor Anasystis nidulans exhibited a significantly higher rate of growth. Variation of the concentration of sodium acetate (10-50 mM) was without effect and attempts to grow A . variabilis in the presence of sodium acetate and absence of carbon dioxide were unsuccessful. The failure of acetate and other substrates to stimulate respiration in blue-green algae has been known for some time (Kratz & Myers, 1955b) and in our experiments A. variabilis was no exception. Over a range of pH values (4-3-8.0) the endogenous rate of respiration of this organism was not significantly affected by the addition of sodium acetate to a concentration of 30 ]TIM. The oxygen uptake by A . nidulans was slightly stimulated by the addition of glucose. Comparison of respiratory rates in the mineral salt medium and 0.1 M-potassiutn phosphate buffer (pH 7.0) showed that the growth medium was a superior environment for respiration by suspensions of washed organisms. When the organisms were starved of carbon dioxide for 4 hr immediately before harvesting, the rate of endogenous respiration was decreased ; the rate of respiration was largely restored by the addition of sodium acetate or glucose; other organic compounds had less effect (Table I ) .
__________________
Cliff Babcock Intestests: Digital Microscopy; Marine Pest Control; Marine Plants & Macroalgae Current Tank Info: 180 g. mixed reef system |
12/03/2011, 07:05 PM | #13 |
Team RC Member
Join Date: Aug 2008
Location: Highland, Maryland Entomologist
Posts: 14,591
|
In this study ethanol stimulates the growth rate of cyanobacteria and acetate has no effect on cyano growth rate.
Characterization of an Alcohol Dehydrogenase from the Cyanobacterium Synechocystis sp. Strain PCC 6803 That Responds to Environmental Stress Conditions via the Hik34-Rre1 Two-Component System▿† http://jb.asm.org/content/191/13/4383.full From it: "As shown in Table 2, the enzyme was active toward a wide variety of primary alcohols and their corresponding aldehydes, but neither ketones nor secondary alcohols were effective substrates." Table 2: http://jb.asm.org/content/191/13/4383/T2.expansion.html
__________________
Cliff Babcock Intestests: Digital Microscopy; Marine Pest Control; Marine Plants & Macroalgae Current Tank Info: 180 g. mixed reef system |
12/03/2011, 07:18 PM | #14 |
Team RC Member
Join Date: Aug 2008
Location: Highland, Maryland Entomologist
Posts: 14,591
|
In conclusion:
Unless someone can provide scientific evidence I have not read, I see no reason to dose vodka (ethanol). Rather I have found plenty of advantages for utilizing vinegar (acetate) as the carbon source in the articles listed above. FWIW, the above articles are just a few of the articles I have gone through to come up with this concludsion.
__________________
Cliff Babcock Intestests: Digital Microscopy; Marine Pest Control; Marine Plants & Macroalgae Current Tank Info: 180 g. mixed reef system |
12/03/2011, 07:51 PM | #15 |
Team RC Member
Join Date: Aug 2008
Location: Highland, Maryland Entomologist
Posts: 14,591
|
I would point out that both bacteria and cyanobacteria produce PHA as an energy storage for use when times are lean, so it is only logical to conclude that the Biopellets can be utilized by cyanobacteria. Anaerobic bacteria do produce PHA as well, so I would assume that the anaerobic bacteria can utilize biopellets. The question I have is since the biopellets are a larger molecule than acetate, how well does PHA penetrate into larger bacterial biomasses where anaerobic bacteria exist?
__________________
Cliff Babcock Intestests: Digital Microscopy; Marine Pest Control; Marine Plants & Macroalgae Current Tank Info: 180 g. mixed reef system |
12/03/2011, 11:06 PM | #16 | ||
Registered Member
Join Date: Feb 2007
Location: Canada
Posts: 212
|
Quote:
Quote:
__________________
Stu Retired 500g system... Researching and building a nice and relaxing 65g display |
||
12/03/2011, 11:26 PM | #17 |
Registered Member
Join Date: Jan 2011
Location: Florida, FWB
Posts: 3,389
|
Highland,
You feed my brain.
__________________
180 Mixed Reef SRO-5000 Skimmer Neptune APEX Gold Kessil AP700/ MP60+6105 Kalk+2 part/ Cheato Fuge Current Tank Info: 180 SPS Dominant |
12/04/2011, 07:15 AM | #18 | |
Team RC Member
Join Date: Aug 2008
Location: Highland, Maryland Entomologist
Posts: 14,591
|
Quote:
Denitrification of aquarium water http://www.google.com/patents?hl=en&...page&q&f=false The author's findings were that it does not work well with fine sand like commonly used in reef tanks. Instead this method works better with crushed coral. Personally I would not use crushed coral in a reef tank. According to the author's findings adding crushed coral to a pellet reactor reduces the amount of biopellets you need to use to accomplish the same amount of reduction. This sounds better to me.
__________________
Cliff Babcock Intestests: Digital Microscopy; Marine Pest Control; Marine Plants & Macroalgae Current Tank Info: 180 g. mixed reef system |
|
12/04/2011, 07:18 AM | #19 |
Team RC Member
Join Date: Aug 2008
Location: Highland, Maryland Entomologist
Posts: 14,591
|
I'll take this as a compliment.
You're welcome.
__________________
Cliff Babcock Intestests: Digital Microscopy; Marine Pest Control; Marine Plants & Macroalgae Current Tank Info: 180 g. mixed reef system |
12/04/2011, 07:35 AM | #20 |
Registered Member
Join Date: Feb 2007
Location: Canada
Posts: 212
|
Thanks Cliff
__________________
Stu Retired 500g system... Researching and building a nice and relaxing 65g display |
12/04/2011, 07:41 AM | #21 |
Team RC Member
Join Date: Aug 2008
Location: Highland, Maryland Entomologist
Posts: 14,591
|
You're welcome.
__________________
Cliff Babcock Intestests: Digital Microscopy; Marine Pest Control; Marine Plants & Macroalgae Current Tank Info: 180 g. mixed reef system |
12/04/2011, 08:49 AM | #22 |
Reef Chemist
Join Date: Apr 2001
Location: Arlington, Massachusetts
Posts: 86,233
|
Great finds, Cliff.
__________________
Randy Holmes-Farley Current Tank Info: 120 mixed reef |
12/04/2011, 09:18 AM | #23 |
Team RC Member
Join Date: Aug 2008
Location: Highland, Maryland Entomologist
Posts: 14,591
|
Thanks Randy.
It's always nice to get a compliment from my mentor.
__________________
Cliff Babcock Intestests: Digital Microscopy; Marine Pest Control; Marine Plants & Macroalgae Current Tank Info: 180 g. mixed reef system |
12/04/2011, 12:30 PM | #24 | |
ReefKeeping Mag staff
Join Date: Apr 2006
Location: West Seneca NY
Posts: 27,691
|
Quote:
Naturally occuring amanonox activity happens predominantly at the edge of sulfur reducing anoxic regions which is where the ammonium for the process originates, as I read it. So would you need SO4 reduction and concomitant sulfide and hydrogen sulfide production for anamonox bacteria to play a significant role in a closed system with or without a deep sand bed or plenum? It was first discovered in the large anoxic basin known as the Black Sea and subsequently in some large upwelling systems in the ocean where it is hypothesized that ammonium wells up from the sulfur reducing regions where it is produced along the way to mineralization. The tank water would normally be low in ammonium in a healthy aquarium. since oxidation via the usual aerobic nitrifying process would likely occur first, so it seems a source of ammonium to very hypoxic areas would be needed to encourage anamonox activity . I don't think that is worth the effort or the risks at least at the stage of knowledge now available . The anamonox bacteria involved are very slow growers(11 to 12 days for division) as far as bacteria go so any efect on nitrogen reduction would likely be very slow. It's difficult to cross over waste water applications to biotypes like aquariums but the studies can be thought provoking .Personally, I don't use them as baseline material for aquariums. Now for winners and losers, vodka and vinegar vs vinegar only. IMO, it's a tie. Ethanol oxidizes to acetic acid(vinegar without the dilution) ,so it sources acetate after an extra step or two as well . These steps don't result in discernible harm and may or may not be of benefit. I'd guess they cause no significant benefit or harm based on my 3 year experience. I'll have to read the study again because I didn't find a clear indication that ethanol suppresses anamonox bacteria but even if it does I'm not sure it matters in an aquarium since there may not be any significant number of them to start with. Ethanol is also a neutral ph additive as opposed to vinegar with a low ph and CO2 additions. I prefer bolus dosing as opposed to incremental dosing via dosing pump and timers needed with larger amounts of vinegar to mitigate precipitous ph effects. Bouls dosing is the routine I've followed for 3 years with satisfying results, a complete change over would change bacterial populations . While I'm always looking for opportunities for improvement as per the shift to 25% vinegar from all vodka several years ago, a total change of carbon source and resuting changes in the bacterial cultures in my aquarium are not a hand I want to play right now.. I also dose some vodka at night without worry about introducing more CO2. Bolus dosing may encourage more anaerobic denitrifiction activity than incremental dosing according to one study. I haven't seen anything that would ease my reluctance to switch to only vinegar in my system in the amnamonox writings but I think vinegar only makes good sense for some systems and would be inclined to try it if I was just starting if for no other reason than to skip the extra bacterial steps ethanol needs to go to acetate andto see how the animals in t4h tank reacted vs the curent vodka/ vinegar method. Vodka does cost more but not much when you don't count the extra water. The vodka I use is $14 for 1.75 liters(about 59 ounces) ,40% of that is ethanol roughly 23.6 ounces ,for a per ounce cost of 59 cents. I use the store brand vinegar in the economy size,It;s the cheapest around here @ $4 for 154 ounces, 95% of which is water leaving 7.7 ounces of acetic acid for $4 or 52 cents per ounce.
__________________
Tom Current Tank Info: Tank of the Month , November 2011 : 600gal integrated system: 3 display tanks (120 g, 90g, 89g),several frag/grow out tanks, macroalgae refugia, cryptic zones. 40+ fish, seahorses, sps,lps,leathers, zoanthidae and non photosynthetic corals. |
|
12/04/2011, 12:32 PM | #25 |
Registered Member
Join Date: Nov 2006
Location: Dingmans Ferry, PA
Posts: 1,083
|
Great stuff Cliff. It sure seems like dosing acetate is the way to go. Using a dosing pump or like you said just adding it to your top off makes it every bit as convenient as bio-pellets.
__________________
Bob Current Tank Info: 200gal system, 150gal & 58gal reef, ATI Powermodule & Sunpower , ATB Deluxe skimmer, KZ zeo reactor, Geo 618, FX1500 return, Tunze 7095, Tunze Streams. |
|
|
Similar Threads | ||||
Thread | Thread Starter | Forum | Replies | Last Post |
some interesting articles about bacteria. | clkwrk | Advanced Topics | 1 | 06/24/2006 11:19 AM |
Interesting article! | Jorsan | Mark van der Wal | 10 | 07/10/2002 08:48 AM |