My journey using Chloroquine Phosphate begins... - Page 66

lagatbezan · 02/22/2018, 04:25 PM

Quote:

Originally Posted by ca1ore

Haven't been on this thread in a while ..... but I seem to recall that CP was a single dose, not a daily dose? I do think with some of these potentially dodgy sources you never really know what you are getting. CP is an accepted med for malaria in humans .....

Yes, you are correct. Its a dose once and thats it. Except when adjusting for water changed.
Also agree with the fact thats important where you get the CP from. Best place to get it is from diamondback_com since they test every batch for purity and the ones purchased from ebay or other places could not really be what they say it is or not in the best pure form.

MrsBugmaster · 03/01/2018, 10:21 AM

Does CP evaporate as water evaporates? I currently have about a half gallon a day water evaporate from QT tank. I keep topping off everyday and just wanted to make sure cp doesn't evaporate too. Is there any way to test for cp in the water?

Dmorty217 · 03/01/2018, 10:27 AM

Quote:

Originally Posted by MrsBugmaster

Does CP evaporate as water evaporates? I currently have about a half gallon a day water evaporate from QT tank. I keep topping off everyday and just wanted to make sure cp doesn't evaporate too. Is there any way to test for cp in the water?

No the CP won't evaporate

bnumair · 03/02/2018, 06:30 AM

not aware of any testing kits or methods for CP

Dmorty217 · 03/02/2018, 06:57 AM

You can test for it with a spectrophotometer. I was advised to buy a Hach spectrophotometer but they aren't cheap. Needless to say I still can't test for CP. Also I would imagine there is a fairly steep learning curve to the instrument if you have never used one.

Louis Z · 03/02/2018, 11:30 AM

Dennis 30 , I too have had issues with cp . I had some Bangii cardinals that succumbed . I feel that cp was more the cause . That’s one reason why I do ttm additionally. I had problems with hypo not doing well with cardinals also . But that maybe just my issues . When I dose cp I try to do the 40 instead of the 80mg/gal and do TTM during the treatment time span with quick FW dips in between transfers . Seems overkill but I like the lower dose and the way TTM is not medication . I do all three if I know if I have ick . If I don’t see ick the I go wth TTM and FW dips in between .

tkeracer619 · 08/30/2018, 04:58 PM

Does anyone have true USP CP from a compounding pharmacy? Can you PM me?

I bought the equipment needed to test CP.

tkeracer619 · 08/31/2018, 12:41 AM

Quote:

Originally Posted by tkeracer619

Does anyone have true USP CP from a compounding pharmacy? Can you PM me?

I bought the equipment needed to test CP.

Ok... after a few trial runs I'm confident this is testing properly. My first chart... green line is what my tank measured. At 25mg/Gal it's about half of what I would have expected.

If someone can get me some known pure USP grade CP I'll be able to create an accurate slope and then I'll be able to measure the purity of these random sources. Let's stop guessing about our CP concentrations. Enough running in circles.

ganeshremedies · 02/13/2019, 06:13 AM

to remove any metabolites or degraded chemical, which may be in solution, is recommended. Also, since phosphate is part of the compound's formula, the use of this compound will tend to increase the concentration of this undesirable algae nutrient, if by no other reason than default. Besides a water change, the use of phosphate-removing media may be required after the treatment has ended. And as with most chemicals, activated carbon should be placed back in the filter to remove the remaining drug from solution, and to fully terminate the treatment.

Dmorty217 · 02/13/2019, 06:56 AM

Quote:

Originally Posted by tkeracer619

Ok... after a few trial runs I'm confident this is testing properly. My first chart... green line is what my tank measured. At 25mg/Gal it's about half of what I would have expected.

If someone can get me some known pure USP grade CP I'll be able to create an accurate slope and then I'll be able to measure the purity of these random sources. Let's stop guessing about our CP concentrations. Enough running in circles.

You will need to go to the local vet and tell them that you need a prescription for your fish for ich and tell them you need CP. There is a company diamond back or diamond something out of Arizona that will provide you 99% pure CP that they get from a place in Canada and they test each and every batch that they get and send it back if it doesn't meet their standards. You could PM Humblefish and get the actual name of the place I am talking about. My memory isn't the best so I apologize

lagatbezan · 02/13/2019, 11:20 AM

I just got an order filled from diamondback that came in. PM me and I will provide you with a sample.

HumbleFish · 02/13/2019, 07:17 PM

Just wanted to add a few observations to this thread...

1. I'm not a chemist, but I have a friend who is and he tests my Chloroquine. He has informed me that a spectrophotometer is not really suitable for testing CP because the photometer "sees" everything that absorbs UV light of a certain wavelength. So it can not distinguish between actual CP and (most likely ineffective) metabolites present in the water.

2. He uses a HPLC with UV detection to test Chloroquine in the water. That equipment is ~ 50k USD.

3. Diamondback Drugs sell pharmaceutical grade (99% pure) CP, but they also require a vet Rx: https://www.diamondbackdrugs.com/

4. Backup plan would be CP from one of the two sources below. Their CP has tested 94-96% pure, which is adequate for our purposes. Just overdose slightly to compensate.

https://www.fishchemical.com/FULL-PR...2937.Item.html

https://store.nationalfishpharm.com/...uine-phosphate

ganeshremedies · 02/25/2019, 04:23 AM

Further research led to finding some public aquariums experimenting with it and having very good success treating the above-mentioned maladies, including Uronema. Early experiments with the product showed that while much safer and less toxic than other traditional treatments of the past, it did have a negative effect on alga. Therefore, aquarium alga and invertebrates containing zooxanthellae were at risk, which were witnessed firsthand in some early experiments, where some soft and stony corals perished (L. Ichinotsubo, pers. com.). Nevertheless, Richard Terrell of the Pittsburgh Zoo Aquarium & PPG Aquarium has reported using chloroquine-dosed gel foods to halt an outbreak of Cryptocaryon in a giant clam exhibit, with excellent results and credits Robyn Doege of the Dallas World Aquarium for providing the information.

In a preparation of the gel food that is made at the aquarium, Rich states: "I used our Metronidazole gel recipe to arrive at an approximation for the Chloroquine. Metro is mixed at 625mg/100g gel diet to yield 25mg/Kg body weight. Chloroquine is supposed to be 50mg/Kg. So I added 3.7g of Chloroquine phosphate to 300g gel powder. Then I added hot water to make the consistency I want. I estimated about 100-125g of fish in the exhibit. Figuring a little less than one part gel powder and medication mixture per one part water by weight, I arrived at about 4 g of gel food per feed. For the garlic gel I mixed about a third of one container of garlic powder into roughly 300g gel powder. Then I added hot water and mixed."

ganeshremedies · 02/25/2019, 04:24 AM

Further research led to finding some public aquariums experimenting with it and having very good success treating the above-mentioned maladies, including Uronema. Early experiments with the product showed that while much safer and less toxic than other traditional treatments of the past, it did have a negative effect on alga. Therefore, aquarium alga and invertebrates containing zooxanthellae were at risk, which were witnessed firsthand in some early experiments, where some soft and stony corals perished (L. Ichinotsubo, pers. com.). Nevertheless, Richard Terrell of the Pittsburgh Zoo Aquarium & PPG Aquarium has reported using chloroquine-dosed gel foods to halt an outbreak of Cryptocaryon in a giant clam exhibit, with excellent results and credits Robyn Doege of the Dallas World Aquarium for providing the information.

In a preparation of the gel food that is made at the aquarium, Rich states: "I used our Metronidazole gel recipe to arrive at an approximation for the Chloroquine. Metro is mixed at 625mg/100g gel diet to yield 25mg/Kg body weight. Chloroquine is supposed to be 50mg/Kg. So I added 3.7g of Chloroquine phosphate to 300g gel powder. Then I added hot water to make the consistency I want. I estimated about 100-125g of fish in the exhibit. Figuring a little less than one part gel powder and medication mixture per one part water by weight, I arrived at about 4 g of gel food per feed. For the garlic gel I mixed about a third of one container of garlic powder into roughly 300g gel powder. Then I added hot water and mixed."
Cyclopropanecarbonitrile-CAS NO.- 5500-21-0 |Methyl cyclopropanecarboxylate-CAS NO.- 2868-37-3 |Cyclopropanecarboxamide-CAS NO.- 6228-73-5 |2-Furoic acid-CAS NO.- 88-14-2 |1-(2-Chloroethyl)pyrrolidine hydrochloride-CAS NO.- 7250-67-1 |thien-4-yl-piperazine-Monohydrochloride]1-Benzo[b]thien-4-yl-piperazine Monohydrochloride-CAS NO.- 913614-18-3 |7-(4-Bromobutoxy)-3,4-dihydro-2(1H)-quinolinone-CAS NO.- 129722-34-5 |Tyramine Hydrochloride-CAS NO.- 60-19-5 |1,2-Cyclohexanedicarboxylic acid-CAS NO.- 1687-30-5 |Dibenzosuberone-CAS NO.- 1210-35-1 |Methyl 4-chlorobutyrate-CAS NO.- 3153-37-5 |

Vinny Kreyling · 12/17/2019, 02:19 PM

Does anyone know the dosage when used as an algaecide?
Thanks

HumbleFish · 12/17/2019, 04:42 PM

Quote:

Originally Posted by Vinny Kreyling

Does anyone know the dosage when used as an algaecide?
Thanks

20mg/gal ought to do it. Chloroquine is a strong algaecide even at low levels.

Vinny Kreyling · 12/17/2019, 04:51 PM

Thank you.

03/01/2018, 10:21 AM	#1627
MrsBugmaster Registered Member Join Date: Nov 2008 Location: Indiana Posts: 182	Does CP evaporate as water evaporates? I currently have about a half gallon a day water evaporate from QT tank. I keep topping off everyday and just wanted to make sure cp doesn't evaporate too. Is there any way to test for cp in the water? __________________ Mom of Army Hero 06 20 2011 Never Forgotten / Always Remembered Current Tank Info: Red Sea Reefer 170, my reef tank. 180 gal FOWLR tank

03/02/2018, 06:30 AM	#1629
bnumair Dr. Reef at ur service Join Date: May 2009 Location: Tulsa, Ok, USA Posts: 7,751 Blog Entries: 15	not aware of any testing kits or methods for CP __________________ Water Quality: NO3 0,Phos 0,Cal 440,Alk 7.5,Mag 1300 "Reef Fast, You Crash, Reef Slow, You Pass" Mike's Reef 3:16 Current Tank Info: 350g DT,95g sump, 50g Frag tank, 4800gph return 4x Sea swirls. 6x AI Vega Color. 200# Pukani rock, dual recirculating skimmer, Biopellet, GFO Carbon rx's, Cal rx. Closed loop. 1.5hp chiller, genesis renew. Apex & RKE

03/02/2018, 06:57 AM	#1630
Dmorty217 Saltwater Addict Join Date: Jan 2012 Location: Vandalia OHIO Posts: 11,624	You can test for it with a spectrophotometer. I was advised to buy a Hach spectrophotometer but they aren't cheap. Needless to say I still can't test for CP. Also I would imagine there is a fairly steep learning curve to the instrument if you have never used one. __________________ Fish are not disposable commodities, but a worthwhile investment that can be maintained and enjoyed for many years, providing one is willing to take the time to understand their requirements and needs Current Tank Info: 625g, 220g sump, RD3 230w, Vectra L1 on a closed loop, 3 MP60s, MP40. Several QTs

08/30/2018, 04:58 PM	#1632
tkeracer619 Registered Member Join Date: Jan 2006 Location: Westminster, CO Posts: 17,289	Does anyone have true USP CP from a compounding pharmacy? Can you PM me? I bought the equipment needed to test CP. __________________ Hobby Experience: 9200ish gallons, 26 skimmers, and a handful of Kent Scrapers. Current Tank: Vortech Powered 600G SPS Tank w/ 100gal frag tank & 100g Sump. RK2-RK10 Skimmer. ReefAngel. Radium 20k.

02/13/2019, 06:13 AM	#1634
ganeshremedies Registered Member Join Date: Feb 2019 Location: Plot No. 6011, G.I.D.C Estate, Ankleshwar, Gujarat, India - 393002. Posts: 3	to remove any metabolites or degraded chemical, which may be in solution, is recommended. Also, since phosphate is part of the compound's formula, the use of this compound will tend to increase the concentration of this undesirable algae nutrient, if by no other reason than default. Besides a water change, the use of phosphate-removing media may be required after the treatment has ended. And as with most chemicals, activated carbon should be placed back in the filter to remove the remaining drug from solution, and to fully terminate the treatment. __________________ thanks Current Tank Info: ganeshremedies

03/02/2018, 11:30 AM	#1631
Louis Z Registered Member Join Date: Apr 2002 Location: Montgomery Posts: 1,257	Dennis 30 , I too have had issues with cp . I had some Bangii cardinals that succumbed . I feel that cp was more the cause . That’s one reason why I do ttm additionally. I had problems with hypo not doing well with cardinals also . But that maybe just my issues . When I dose cp I try to do the 40 instead of the 80mg/gal and do TTM during the treatment time span with quick FW dips in between transfers . Seems overkill but I like the lower dose and the way TTM is not medication . I do all three if I know if I have ick . If I don’t see ick the I go wth TTM and FW dips in between .

02/13/2019, 11:20 AM	#1636
lagatbezan Registered Member Join Date: Nov 2004 Location: Long Island, NY Posts: 1,109	I just got an order filled from diamondback that came in. PM me and I will provide you with a sample. __________________ Ryan. 300g with velocity t4 for return and a Reflo dart on a closed loop, T5 lights & SRO3000 skimmer. Current Tank Info: 300g

02/13/2019, 07:17 PM	#1637
HumbleFish Moved On Join Date: Feb 2013 Posts: 4,757	Just wanted to add a few observations to this thread... 1. I'm not a chemist, but I have a friend who is and he tests my Chloroquine. He has informed me that a spectrophotometer is not really suitable for testing CP because the photometer "sees" everything that absorbs UV light of a certain wavelength. So it can not distinguish between actual CP and (most likely ineffective) metabolites present in the water. 2. He uses a HPLC with UV detection to test Chloroquine in the water. That equipment is ~ 50k USD. 3. Diamondback Drugs sell pharmaceutical grade (99% pure) CP, but they also require a vet Rx: https://www.diamondbackdrugs.com/ 4. Backup plan would be CP from one of the two sources below. Their CP has tested 94-96% pure, which is adequate for our purposes. Just overdose slightly to compensate. https://www.fishchemical.com/FULL-PR...2937.Item.html https://store.nationalfishpharm.com/...uine-phosphate

02/25/2019, 04:23 AM	#1638
ganeshremedies Registered Member Join Date: Feb 2019 Location: Plot No. 6011, G.I.D.C Estate, Ankleshwar, Gujarat, India - 393002. Posts: 3	Further research led to finding some public aquariums experimenting with it and having very good success treating the above-mentioned maladies, including Uronema. Early experiments with the product showed that while much safer and less toxic than other traditional treatments of the past, it did have a negative effect on alga. Therefore, aquarium alga and invertebrates containing zooxanthellae were at risk, which were witnessed firsthand in some early experiments, where some soft and stony corals perished (L. Ichinotsubo, pers. com.). Nevertheless, Richard Terrell of the Pittsburgh Zoo Aquarium & PPG Aquarium has reported using chloroquine-dosed gel foods to halt an outbreak of Cryptocaryon in a giant clam exhibit, with excellent results and credits Robyn Doege of the Dallas World Aquarium for providing the information. In a preparation of the gel food that is made at the aquarium, Rich states: "I used our Metronidazole gel recipe to arrive at an approximation for the Chloroquine. Metro is mixed at 625mg/100g gel diet to yield 25mg/Kg body weight. Chloroquine is supposed to be 50mg/Kg. So I added 3.7g of Chloroquine phosphate to 300g gel powder. Then I added hot water to make the consistency I want. I estimated about 100-125g of fish in the exhibit. Figuring a little less than one part gel powder and medication mixture per one part water by weight, I arrived at about 4 g of gel food per feed. For the garlic gel I mixed about a third of one container of garlic powder into roughly 300g gel powder. Then I added hot water and mixed." __________________ thanks Current Tank Info: ganeshremedies

02/25/2019, 04:24 AM	#1639
ganeshremedies Registered Member Join Date: Feb 2019 Location: Plot No. 6011, G.I.D.C Estate, Ankleshwar, Gujarat, India - 393002. Posts: 3	Further research led to finding some public aquariums experimenting with it and having very good success treating the above-mentioned maladies, including Uronema. Early experiments with the product showed that while much safer and less toxic than other traditional treatments of the past, it did have a negative effect on alga. Therefore, aquarium alga and invertebrates containing zooxanthellae were at risk, which were witnessed firsthand in some early experiments, where some soft and stony corals perished (L. Ichinotsubo, pers. com.). Nevertheless, Richard Terrell of the Pittsburgh Zoo Aquarium & PPG Aquarium has reported using chloroquine-dosed gel foods to halt an outbreak of Cryptocaryon in a giant clam exhibit, with excellent results and credits Robyn Doege of the Dallas World Aquarium for providing the information. In a preparation of the gel food that is made at the aquarium, Rich states: "I used our Metronidazole gel recipe to arrive at an approximation for the Chloroquine. Metro is mixed at 625mg/100g gel diet to yield 25mg/Kg body weight. Chloroquine is supposed to be 50mg/Kg. So I added 3.7g of Chloroquine phosphate to 300g gel powder. Then I added hot water to make the consistency I want. I estimated about 100-125g of fish in the exhibit. Figuring a little less than one part gel powder and medication mixture per one part water by weight, I arrived at about 4 g of gel food per feed. For the garlic gel I mixed about a third of one container of garlic powder into roughly 300g gel powder. Then I added hot water and mixed." Cyclopropanecarbonitrile-CAS NO.- 5500-21-0 \|Methyl cyclopropanecarboxylate-CAS NO.- 2868-37-3 \|Cyclopropanecarboxamide-CAS NO.- 6228-73-5 \|2-Furoic acid-CAS NO.- 88-14-2 \|1-(2-Chloroethyl)pyrrolidine hydrochloride-CAS NO.- 7250-67-1 \|thien-4-yl-piperazine-Monohydrochloride]1-Benzo[b]thien-4-yl-piperazine Monohydrochloride-CAS NO.- 913614-18-3 \|7-(4-Bromobutoxy)-3,4-dihydro-2(1H)-quinolinone-CAS NO.- 129722-34-5 \|Tyramine Hydrochloride-CAS NO.- 60-19-5 \|1,2-Cyclohexanedicarboxylic acid-CAS NO.- 1687-30-5 \|Dibenzosuberone-CAS NO.- 1210-35-1 \|Methyl 4-chlorobutyrate-CAS NO.- 3153-37-5 \| __________________ thanks Current Tank Info: ganeshremedies

12/17/2019, 02:19 PM	#1640
Vinny Kreyling Registered Member Join Date: Nov 2002 Location: Miller Place, NY Posts: 7,206	Does anyone know the dosage when used as an algaecide? Thanks __________________ 250 gallon mixed reef, 2 Reefbreeder's Photon V 2, Deepwater BLDC 12, DAS EX-3 Skimmer, MTC mini cal, 2-3/4" Sea Swirls, Aquacontroller & 6 Tunze pumps.

12/17/2019, 04:51 PM	#1642
Vinny Kreyling Registered Member Join Date: Nov 2002 Location: Miller Place, NY Posts: 7,206	Thank you. __________________ 250 gallon mixed reef, 2 Reefbreeder's Photon V 2, Deepwater BLDC 12, DAS EX-3 Skimmer, MTC mini cal, 2-3/4" Sea Swirls, Aquacontroller & 6 Tunze pumps.