|04/18/2012, 06:31 PM||#1|
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Join Date: Aug 2005
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Interesting Facts about Cryptocaryon Irritans
Interesting facts about Cryptocaryon irritans Infections
Cryptocaryon irritans (Cryptocaryon) is a ciliated, protozoan parasite which causes a disease known as marine "ich" or marine "white spot" disease in wild and cultured marine fishes at temperatures between 59–86°F. (Burgess and Matthews 1995; Diggles and Lester 1996a; Colorni and Burgess 1997). This will provide some hopefully interesting answers to some of the questions that have come up over time in the Reef Central Fish Diseases Forum.
Cryptocaryon is known to infect many different fish species, although there appear to be differences in susceptibility (Wilkie and Gordin 1969; Colorni 1985; Colorni and Burgess 1997). I tend to see Tangs, butterfly fish, and angels infected more than most other species of fish. On the other hand mandarins and other fish with a strong slime coating are rarely if ever affected.
Strain differences among different Cryptocaryon isolates from various parts of the world have been identified, and, although many have similar life cycles and salinity tolerances, others have been found to be outside previously described "normal" ranges (Yambot et al. 2003). That is one of several reasons I hesitate to suggest hyposalinity as a treatment option.
Signs of infection, complex life cycle stages, and the explosiveness with which infection and deaths can occur—often within days in culture situations—(Colorni and Burgess 1997) are similar in many ways to those seen for the freshwater parasite Ichthyophthirius multifiliis (Floyd and Reed 2009). However, the two are only distantly related, and major differences exist with regard to salinity tolerance and duration of life cycle.
Accurate diagnosis and rapid response and treatment are necessary to reduce losses, which can be devastating.
Signs of Disease
Fish infected with Cryptocaryon will often have small white spots, nodules, or patches on their fins, skin, or gills but visual signs are not a requirement . They may also have ragged fins, cloudy eyes, pale gills, increased mucus production, or changes in skin color, and they may appear thin (Noga 1996; Colorni and Burgess 1997). Because the characteristic white spots might not be obvious in pale-colored fish or may not appear at all in infections with only gill involvement, absence of "white spots" or nodules—or even parasites—on the fin or skin does not rule out Cryptocaryon. Behavior is usually a good indicator
Behaviorally, fish may flash (scratch), swim abnormally, hang at the surface or on the bottom, act lethargic, or breathe more rapidly as if in distress (Colorni and Burgess 1997). Within a population, mortalities may increase rapidly over the course of several days. However, the extent of pathology will differ depending upon the strain of parasite, the species of fish, previous exposure to the parasite, and the temperature of the water. While lower temperature raise dissolved oxygen level in the water, it also prolongs the life cycle.
Biology and Life Cycle of Cryptocaryon irritans
This information is summarized in a sticky in this forum. The length of the entire life cycle varies, depending upon a number of factors, including strain of Cryptocaryon, temperature, salinity, and fish host (Colorni 1985; Diggles and Lester 1996a, b, c; Colorni and Burgess 1997; Yambot et al. 2003). Even for a specific strain and fish host, the life cycle may vary by weeks or months (Colorni and Burgess 1997). An average life cycle appears to be 1 to 2 weeks; however, life cycle durations may range from 6 days to 11 weeks, primarily because of the unpredictability of tomont development (Colorni and Burgess 1997; Dickerson 2006; Yambot 2003). I normally encourage a minimum of 9 weeks of a tank being fallow in order to eliminate the parasite; obviously 12 weeks would be better still.
In addition, some characteristics of the different life cycle stages (e.g., size and time required for development) vary depending upon the strain of Cryptocaryon irritans, the salinity and temperature of the water, and the species of fish infected (Diggles and Lester 1996 a, b, c).
Temperatures for optimal growth of most strains of Cryptocaryon appear to be about 73.4–86°F (Dickerson 2006; Yoshinaga 2001), although active infections at 59°F have been documented (Diggles and Lester 1996). Encysted stages, off the host (tomonts), were also observed to survive for 2–4 weeks under experimental hypoxic conditions (24% oxygen saturation); these released free-swimming infective stages (theronts) 10–11 days after excystment (Yoshinaga 2001).
A more recent study demonstrated that two life stages of one strain of Cryptocaryon (trophonts, i.e. the feeding stage during which the parasite can be found on the fish, and tomonts) survived dormant for 4–5 months at 53.6°F, and, after the water temperature increased to 80.6°F, developed and infected fish (Dan et al. 2009).
The most commonly observed stage is the trophont, or "feeding" stage. The trophont is found on the fish, usually underneath the outer skin layers. Spherical to club- or pear-shaped, with cilia all over its body, the trophont will normally be seen "rolling" or rotating slowly under the epithelium (outer cell layers of the skin or gills) (see figures 3–6 ). Because the trophont is embedded within the skin, it is relatively protected from any potential treatments. The organism's cytoplasm is more opaque in this phase, which means the lobed macronucleus (and several smaller micronuclei) can be difficult to see in live specimens (Dickerson 2006). Trophonts can range in size from about 48 x 27 µm (~1/20 x 1/40 of a mm) to 452 x 360 µm (~1/2 x 1/3 mm) (1 µm = 1 micron; 1,000 microns = 1 millimeter). The trophont feeds on the body fluids and cells of the fish for about 3–7 days before leaving the host.
Trophonts will also actively leave fish that have died, but are not immediately infective. They require additional time to develop from protomonts to tomonts, just as they would if leaving a live host.
Once a trophont leaves the fish, it becomes a protomont. During this phase, it loses its cilia, flattens its surfaces, and moves onto a substrate for about 2–18 hours. After this stage, the organism stops, sticks to the surface, and encysts, whereupon it becomes a tomont. The cyst hardens in about 8–12 hours (Colorni 1985). Before the cyst forms, the protomont may be susceptible to some treatments for a short period of time. However, once the cyst has formed and hardened around the tomont, it has greater protection against common treatments
Tomonts range in size from 94.5 x 170 µm (~ 1/10 mm x 1/6 mm) to 252 x 441 µm (~1/4 x 1/2 mm). The tomont of one strain of Cryptocaryon was 210 x 763 μm (~1/5 x 3/4 mm). The encysted tomont undergoes many divisions, producing numerous daughter tomites (approximately 100 to 1000, depending upon strain and temperature [Colorni and Burgess 1997]). These tomites are released as theronts, the free-swimming infective stage which is also the stage most susceptible to most salinity or chemical treatments.
The time required for theront development varies. In one study (Colorni and Burgess 1997), theronts emerged from a group of tomonts sometime between 3 and 72 days, with most released from 4 to 8 days after tomont formation. In another study (Diggles and Lester 1996c ), tomite development and theront release occurred, on average, between 5 and 12.1 days after tomont formation, depending upon strain and temperature. There was no correlation between tomont size and theront release.
Yoshinaga and Dickerson (1994) observed, in laboratory studies, that theronts were released only between the hours of 2:00 am and 9:00 am, even in total darkness; some suggest this strategy increases the chance for theronts to find a host, as many fish may be resting or closer to substrate during this time period. This is why I always suggest moving fish during tank transfer quarantine protocols in the morning.
Theronts are oval to pear-shaped and motile; they actively seek fish. The theront is the most exposed, unprotected life stage and therefore the most logical target for treatment. Once the theront locates a host, it invades its skin within 5 minutes (Dickerson 2006). During gill invasion, the parasite becomes enclosed by a thin layer of cells within 20–30 minutes (Dickerson 2006). Theronts of one strain were 20–30 x 50–70 µm (Colorni 1985), but size will vary depending upon strain, host species, and temperature. The theront's infectivity is highest early in its life. By 6–8 hours after it leaves the cyst, its infectivity is greatly reduced (Burgess 1992; Yoshinaga and Dickerson 1994; Colorni and Burgess 1997; Dan et al. 2009), although a non-infective theront may still be able to move for up to 48 hours.
As is seen with other diseases, general fish health and environmental factors including water quality will affect the status of the fish's immune system and may worsen the effects of an infection. If the immune status of the fish is compromised or if environmental factors are less than optimal, Cryptocaryon infection will be even more explosive and harmful. People that advocate boosting the immune system make survival more probable, but this does not rid the tank of Cryptocaryon irritans.
Fish that survive a Cryptocaryon infection develop immunity to that particular strain of Cryptocaryon, which can prevent significant disease reoccurrence for up to 6 months (Burgess 1992; Burgess and Matthews 1995). However, these survivors may act as carriers and provide a reservoir for future outbreaks (Colorni and Burgess 1997).
More targeted development of a vaccine to protect against Cryptocaryon irritans has been ongoing for a number of years (Yambot and Song 2006; Hatanaka 2007; Luo et al. 2007; Bai et al. 2008), and preliminary results are encouraging. However, vaccine development is a lengthy process, and no commercial vaccines are currently available.
Prevention and Control
An understanding of Cryptocaryon's life cycle provides a scientific framework for disease prevention and management. The ultimate goal of a prevention or control program is to break the life cycle of the parasite and stop future infections.
How long each life stage will need for development will depend upon the fish species affected, the fish's immune status, the strain of Cryptocaryon, and environmental factors including temperature and salinity. However, the wide variability and length of the Cryptocaryon life cycle, and in particular, the time required for tomite development and theront release; the presence of protected, "embedded" and encysted stages on and off the fish; and the potentially devastating consequences of an outbreak of this infection necessitate a prolonged quarantine and treatment period. A minimum quarantine period of 3–6 weeks at 75.2–80.6°F, is advised, and longer time frames (e.g., 7–11 weeks) may be necessary.
The water in the affected system must be treated in some way to kill the theronts living in the water column. Likewise, substrate (including, possibly, parts of the fish) may harbor encysted tomonts, and therefore tanks and associated substrate and materials will act as "incubators" of Cryptocaryon. Clean or replace these to help to reduce reinfection.
Use of ultraviolet (UV) sterilization to kill theronts has been suggested, based on research involving Ichthyophthirius multifiliis (freshwater "ich"). The recommended UV dose for Ichthyophthirius theronts is 100,000 µWsec/cm2 (Hoffman 1974). However, UV doses required for Cryptocaryon irritans are anecdotal or extrapolated, and range from 280,000 µWsec/cm2 (industry numbers) to 800,000 µWsec/cm2 (Colorni and Burgess 1997).
Theronts must go through the UV sterilization unit in order to be exposed, so any theronts that are not exposed to UV radiation and remain in the tank or holding areas will be unaffected. Similarly, encysted tomonts within the tank or holding area will not be affected. UV is not a bad thing but it is not a solution to this problem. However if water is shared among tanks usage of UV will isolate other tanks from infection.
Ozonation is a highly effective method for disinfection of water, but is more complicated and may affect water quality, especially with regard to reaction products in salt water. There is limited information on doses required to kill theronts or other life stages (Colorni and Burgess 1997).
Although generally not feasible for large populations, large or complex systems, or weak or debilitated fish, one approach that has been suggested is transfer of affected fish into new, bare bottom tanks every 3 days (Colorni 1987). Tanks are cleaned, disinfected (see Disinfection below), and dried between moves. This approach reduces or prevents tomont development on the substrates, and subsequent reinfection. Depending upon temperature, fish may need to be moved 3–5 times. For normal aquarium temperature 4 times should be sufficient. This is the option I recommend.
Several chemical treatment options have been used most commonly and effectively against Cryptocaryon in marine aquaria and aquaculture systems. Most treatments target the free-swimming theront. Standard immersion (bath) treatments include copper, considered by many to be the most effective; hyposalinity (reducing salt concentration); and chloroquine. Formalin, though less commonly described in the literature to treat Cryptocaryon, has also been used with varying success.
Copper, in the form of copper sulfate pentahydrate (CuSO4•5H20; the "blue" copper), is perhaps the most commonly used chemical against external protistan (protozoal) parasites of marine finfish. When used in marine systems, copper sulfate pentahydrate is considered 25.5% active ingredient (i.e., 25.5% is active, "free copper" (Cu2+).
The recommended dose is 0.15–0.20 mg/L free copper (Cu2+) (Noga 1996); however, this treatment concentration should be attained gradually, preferably over the course of 2–3 days, and careful measurement during dosing is necessary. This "breaking in" period will allow the fish time to increase their ability to reduce the toxic effect of copper (de Boeck et al. 2003). Having and using a good copper test kit is important, because copper levels can fluctuate during treatment. Free copper levels should be checked at least twice a day to ensure that copper levels are within the desired range.
Because of the prolonged life cycle of Cryptocaryon, affected systems should be treated for a minimum of 3–6 weeks (Noga 1996; C. Innis, pers. comm., T. Clauss, pers. comm.). As described above, in some reports at lower temperatures, theronts were not released until 72 days after initial tomont formation, so some situations may require longer treatment time periods.
Chelated copper (copper that has been bound or "complexed" to other substances, such as citrate or EDTA, to increase its stability in water) has also been used, but safety and effectiveness are more variable than with copper sulfate pentahydrate (Noga 1996). If using a commercial product, refer to the manufacturer's directions and/or speak with a company representative for best results.
Hyposalinity refers to exposure of fish to a salt concentration that is lower than that in which they normally live (typical tropical marine systems range between 30–35 g/L (g/L=ppt)). Lower salinities are less easily tolerated by many common marine tropical species, which prefer a tighter range of salinity (stenohaline). Therefore, for many species, the lower the salinity, the shorter the time period the fish can tolerate. Freshwater or lower salinity dips (duration in minutes) or short or prolonged immersion baths (duration in hours or days) for tolerant fish species are commonly used to kill or reduce the numbers of external parasites on marine species. Since Cryptocaryon is deeply embedded, fresh water dips often do not reach and therefore do not affect the parasite.
However, Cryptocaryon has proven to be more challenging to treat using salinity changes. Because trophonts and tomonts are more protected, longer dips and baths will be required than for many other species of parasites. Exposure to freshwater for up to 18 hours did not seem to affect Cryptocaryon trophonts on the host (Colorni 1985). Prolonged exposure to 15–16 g/L salinity or less (Cheung et al. 1979; Colorni 1985) appeared to affect some life stages. Tomonts of one strain of Cryptocaryon were effectively killed after 48 hours of exposure to 15 g/L or less (Colorni 1985). Temperature will also determine whether hyposalinity will control the parasite, with temperatures outside the optimal range (23–30°C) causing greater breakdown of tomonts (Cheung et al. 1979).
More recently, studies have demonstrated different salinity tolerances among strains of Cryptocaryon. Yambot (2003) described one Taiwanese outbreak occurring in sea bream Sparus sarba at a salinity of 5 g/L, and another outbreak in sea perch Lates calcarifer occurring at a salinity of 10 g/L. These two strains were successfully propagated in the laboratory at 7 and 10 g/L, respectively, and are well below previously documented preferred salinities.
One suggested protocol that may have some effectiveness, depending upon temperature and the strain's salinity tolerance, is to maintain water at 15 g/L for 21–30 days (Noga 1996; Kinsler, pers. comm.). Salinity should be reduced gradually by 5 to 10 g/L per day until 15 g/L is reached.
Chloroquine, a quinine derivative, and other related compounds have been recommended for use against Cryptocaryon and other protozoan parasites including Amyloodinium (Dickerson 2006; Stoskopf 1993; Noga 1996; Roberts et al. 2009; I Berzins, pers. comm.; T. Clauss, pers. comm.). One recommended treatment regimen is 10 mg/L chloroquine diphosphate as a prolonged bath; duration of 2 to 3 weeks or more may be required. Chloroquine appears to be fairly stable. If water changes are necessary, redose in amounts proportional to quantity of water removed.
Formalin has been used with variable success and differing dosage regimens (Hoff 1996; Colorni and Burgess 1997; R. Francis-Floyd and D. Petty pers. comm. 2009). One suggested regimen is concurrent hyposalinity (16–18 g/L) and 25 mg/L formalin every other day, for 4 weeks (R. Francis-Floyd and D. Petty pers. comm.). Consider species sensitivities and specific system idiosyncrasies, as well as properties of formalin; be aware of these prior to use (Francis-Floyd 1996).
Use of hyposalinity, drugs, or chemicals during quarantine should also further reduce chance of spread. Trophonts on the fish and encysted stages (tomont) off the fish are well-protected against many common treatments. The free-swimming theronts are considered the most susceptible stage, and are the target of most treatments.
However, keep in mind that carriers may also be present in the established (resident) population and thereby infect any new, naďve fish that have been added to that system, even if these new fish were quarantined.
Cryptocaryon irritans, the causative agent of "marine white spot disease" is an important disease of marine and brackish water finfish, and has been documented in aquacultured, captive display, and wild populations. Common disease signs include white spots or areas of increased mucus, flashing, or respiratory distress; however, other signs may be seen, and fish should be sampled by a fish health specialist to verify presence of the parasite with a microscope. This is infeasible for most aquarists.
A number of factors determine how severe the disease and mortalities will be and the length of the parasite life cycle. These factors include the strain of Cryptocaryon, the temperature and salinity of the water, the species and age of the fish and their general immune status, previous exposure to the parasite, the number of infective parasites present, and the dissolved oxygen concentration of the water. The life cycle can last from 6–11 weeks, but an average parasite life cycle appears to be 1–2 weeks. However, because the time required until release of infective theronts after tomont formation varies so widely, prolonged treatment periods (3–6 weeks) are recommended. Longer treatment time may be necessary.
Treatments recommended here for any given outbreak should provide significant mitigation of disease. Copper sulfate pentahydrate appears to be the most effective treatment to date. Chloroquine and salinity, as well as formalin, have also proven to have some effectiveness. If the Cryptocaryon strain affecting your fish is tolerant of low salinity, however, hyposalinity will not be an effective control.
Quarantine of any new fish for 30–90 days before introducing them into an established population will provide time for observation, treatment, and reduction of spread. Source water should also be considered a potential reservoir, and therefore should be handled appropriately. In addition, any equipment, tank structures, or other inert materials should be disinfected properly prior to reuse in other systems. Clinically healthy fish that have survived an infection may act as carriers. Similarly, slow developing or dormant tomonts in the environment may act as a reservoir.
Warmest regards and best wishes,
It is my policy to not respond to those who ask questions not to learn
but to be bellicose.
Life is a series of decisions serially executed but collectively judged.
"Not using a quarantine tank is like playing Russian roulette. Nobody wins the game, some people just get to play longer than others." - Anthony Calfo
|03/08/2013, 12:50 PM||#2|
Join Date: Nov 2009
Location: Long Island,New York
nice post well done
Experience is the product of mistakes
Its better to have lost one fish in quarantine than all the fish in the display tank!
reef tanks are my heroin and I am a junkie.
Current Tank Info: 480 gallon system 180 display tank recently reset,plumbed to a 110 fuge and another 110 display 2 30 gallon resorvoirs and sump. reseach room presenlty with 6 20 gal 6 29 gal 1 50 gal, tank and growing , culturing micro algae,copepods,rotifers
|06/17/2013, 01:40 PM||#4|
Join Date: Nov 2012
So I have a 60 gallon reef tank and a few weeks ago a big turbo snail died and messed up my params a little bit, then that caused the tank to have an algae bloom. It's been a nightmare.... anyway about two or three days ago I noticed my foxface has white bumps on him. The bumps are about the size of a grain of salt, maybe slightly smaller. his only roommate is coral beauty I added about two months ago who doesn't have any. I was looking at some pics of Cryptocaryon irritans and it doesn't look quite like that. I wanna know if its some kind of a reaction to stress or if I should fear the worst? his behavior hasn't changed at, all his breathing is fine, hes always hungry and he doesn't flash. he just seems normal but, with white dots on his side.