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Old 09/26/2014, 05:12 PM   #1
aquatictec
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Harlequin Shrimp Project

Please see our other post on harlequin shrimp.

We now believe we have a system in place to rear larval harlequin shrimp to adults.

On 09/22/2014 we collected 1926 larvae from our breeding pair of harlequin shrimp
We set up 2 systems one with 326 and one with 1600. After 2 days we realized that we did not have enough food to raise that many. Rather than loose them all to starvation we kept 200 and released the rest in our greenhouse systems to fend for themselves.


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Old 10/08/2014, 07:00 PM   #2
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notes on harlequin shrimp

10/08/2014 day 16

We lost most of the last batch to an accident when someone decided they did not like our strainer because it clogged too quickly so they made their own and most of the shrimp either passed through and were filtered out or got stuck on the strainer. We still have about 20 left and they are doing great.



Last edited by aquatictec; 10/08/2014 at 07:01 PM. Reason: left out day 16
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Old 10/12/2014, 02:31 PM   #3
aquatictec
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update check our facebook page

Check our facebook page:



https://www.facebook.com/AquaticTechnologyOhio

for updates on our research project on harlequin shrimp.
As of October 12 the last batch is 20 days old. We collected a new batch of 892 yesterday and split them with our research partner. We put them in the same system with the surviving members of the last batch.

Harlequin shrimp
captive raised
captive bred


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Old 12/12/2014, 11:33 PM   #4
Fedral144
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We set up 2 systems one with 326 and one with 1600. After 2 days we realized that we did not have enough food to raise that many. Rather than loose them all to starvation we kept 200 and released the rest in our greenhouse systems to fend for themselves.


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Old 12/15/2014, 06:32 PM   #5
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update?


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Old 12/17/2014, 02:32 PM   #6
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Harlequin Shrimp Updates

check our face book page for updates

https://www.facebook.com/AquaticTechnologyOhio

Our research partner using the same procedures as us but with less human error has succeeded in rearing 50 plus larvae to adults.

To date we have not reared any to adults. Our losses have all been attributed to human error:
We did not have enough food
our water quality was poor
our salt level was too high
our strainers ripped several times

we now check and adjust water parameters 3x weekly

we now have 35 gallons of copepod cultures and can reach culture densities of up to 100 copepods per ml but
are still struggling with the food mostly culturing iso for the copepods

For our next batch we are going to install a second 100 micron strainer after the first one in case it tears

On another note,
we have plans for attempting to culture seahorses, cleaner shrimp, and blood red shrimp when our new coral system is up and running
possibly within the next month


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Old 03/09/2015, 10:03 AM   #7
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still trying

We have not been able to keep the larvae longer than about 21 days
I am not sure why.
Our current system has a 55 micron overflow screen. The water trickles into a 15 gallon sump from a 15 gallon bucket with a rounded bottom. We have 2 buckets a black one and a blue one
The sump has a constant height section with a filter sponge and a protein skimmer. Water is drawn through the filter sponge with an airlift

In the other section of the sump there is a phosphate reactor and a return pump which trickles the water back.
We have a heater which we have kept both in the sump and in the bucket. The water has been kept anywhere from 74 to 84 degrees.
The specific gravity is kept between 1.023 and 1.026

air is bubbled lightly into the bottom center of the bucket where it washes upwards over the overflow screen. We have also placed the sponge in the bucket at times. It doesn't appear to capture the larvae.

We tint the water daily with iso to lightly cloud it and feed rotifers and parvo initially and then add parvo daily to maintain 6-12 adults per ml.

The larvae just disappear over time

I make the strainers by drilling a bunch of holes in a 2" piece of pvc and gluing a 55 micron screen obtained from Florida Aqua Farms over it.

The screens have split many times and may be the culprit.
The screens are washed in a dilute bleach solution and rinsed in dechlor and
set aside to dry whenever they start to clog
when they split they are removed and a new one is glued on


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Old 03/16/2015, 04:02 PM   #8
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whats up

We collected about 72 larvae today March 16th.
It appears from the low number that they were born yesterday and we didn't notice them until today.

They went into a previously sterilized system with a blue 15 gallon bucket with rounded corners a heater, a stream of air bubbles in the center under the screened overflow with a new 55 micron screen.
The water is kept at 81 degrees. The heater is kept with the shrimp because it is much easier to regulate the temperature that way since the water from the sump just trickles back.

The 15 gallon sump has a constant height section with protein skimmer and a sponge. The other part of the sump houses a a phosphate reactor and a return pump.


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Old 03/21/2015, 08:42 AM   #9
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Cool project! Needs to be more of these efforts, but having the facilities and patience are taxing. Thank you for attempting this! Good luck and please keep this thread updated. Some of us refuse to open a FB account.


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Old 03/26/2015, 04:44 PM   #10
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I was there

I saw your set up. Good luck!


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Old 04/07/2015, 01:11 PM   #11
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Easter Sunday Spawning of Harlequin Shrimp



On Easter Sunday we had a very large batch of Harlequin Shrimp larvae. Gregory and Wilson are shown in the photo. Gregory is collecting the larvae. In our previous attempts we think the larvae were getting stuck on the strainers so we redesigned them to hopefully avoid this and to make removing and replacing them much easier. We of course were caught off guard and our copepod culture count was a little low so we also gave them some fresh hatch baby brine. Wish us luck!


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Old 04/07/2015, 01:21 PM   #12
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Great project and I applaud you guys for doing it.

I totally agree that some people refuse to operate a Facebook page so relying totally on that is going to leave a lot of people out of the loop.. I have a Facebook and I still don't go to links requiring it just because every time I open Facebook it wants more personal info from me than I'm willing to comfortably give, I think others feel the same way. Just saying it may be better to keep both( Facebook and the largest reef forum in the world) an option if you want the most feedback or qualified opinions/praise.

Thanks for sharing


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Old 04/22/2015, 09:06 PM   #13
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excited to hear some good updates


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Old 04/23/2015, 04:35 PM   #14
aquatictec
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aaargh

We appear to have lost the last batch.
We never found any dead ones on the bottom
We did not notice any dead ones on the strainer.
The new strainers work great
We will look at water movement on the next batch.


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Old 04/24/2015, 01:34 PM   #15
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New Batch

The last batch appears to be gone.
We collected 373 larvae today, April 24th, 2015 and transferred them to a empty larval rearing system.
Everything is the same as last time except this time the tub is blue instead of black and the circulation is turned up higher.


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Old 04/29/2015, 12:02 PM   #16
aquatictec
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We keep killing them faster and faster. The April 24th batch appears to be gone. We did not have enough circulation.
The bubble ring kept producing less bubbles.
We have made a new bubble ring by melting the holes in it instead of puncturing them.


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Old 05/14/2015, 12:23 PM   #17
aquatictec
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new hatch

On 5/13/2015 we collected 46
on 5/14/2015 we collected an additional 83

They are in a sterilized black tub with a new style strainer with a new bubble ring with enough circulation to keep them suspended, with a cycled sponge filter, protein skimmer, and phosphate reactor. Parvo density is kept at 6 + /ml
iso cultures are going good
parvo cultures are going good
all the stars are aligned
maybe this time


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Old 05/14/2015, 12:41 PM   #18
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What are you feeding the parents?


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Old 05/14/2015, 01:33 PM   #19
aquatictec
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asterina starfish and chocolate chip starfish


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Old 07/19/2015, 01:31 PM   #20
aquatictec
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Loss in the family

We lost our male harlequin yesterday. He went to the great shrimp yard in the sky. We are now in search of a new pair. We have another female so 2 males would also work. If anyone local has a male they would like to trade for something let us know.



Last edited by aquatictec; 07/19/2015 at 02:01 PM. Reason: typo
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Old 09/04/2015, 08:07 PM   #21
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Try to include blue linckia into diet.

Use a kreisel utilising water flow and not air.

A person good to consult would be Syd Kraul.


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Old 09/13/2015, 01:52 PM   #22
aquatictec
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The larvae don't settle for 45 plus days. It would be very difficult to build/maintain a Kreisel using a pump since the screens would clog from the small size of the food.

We don't have a Kreisel but by releasing air in the center of a round bucket you create a toroid which has the same effect. Many hatcheries use this method

The air does not SEEM to bother them but I guess you don't know until you try it


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Old 03/27/2016, 08:14 AM   #23
aquatictec
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Harlequin shrimp culture continued

Our first pair of harlequin shrimp has long since passed away
Their offspring are already sexually mature

Our second pair of harlequin shrimp released their first clutch of larvae on March 6th, 2016. They all died within a few days. They did not seem capable of staying in the water column. These are are not second generation They are another wild collected and obtained months apart from each other.

On March 26th they released their second batch of over a thousand
We have them in the same system used on all previous attempts.

I think we have our iso culture down. We hardly ever loose a culture.
Our parvo culture is also doing well.

I am thinking of making a Kreisel tank out of glass or acrylic so we can observe them better.


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Old 03/27/2016, 08:40 AM   #24
aquatictec
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sample culture notes Harlequin shrimp larvae culture

yesterday the culture was fed iso to lightly cloud the water and enough parvo culture to obtain a density of 2.5 3 copepods per ml

This morning the density was down to near zero.
We added 4 600 ml portions or parvo
the density count went up to 4 per ml
the copepods did not appear to be full so we added more iso to the culture
We also strained the iso through a 200 micron felt filter bag (This is a new procedure)

We did not perform a density count on the larvae.
It would be very difficult to estimate their numbers.
They are capable of going wherever they want to and are not evenly distributed


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Old 04/14/2016, 10:51 AM   #25
aquatictec
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Update 4/14/2016

The last batch died at about 5 days
The air ring had come loose

We had a new partial release yesterday 4/13/2016 of about 100.
The rest were released this morning and we are collecting them now.

I think we need to come up with a better means of keeping them suspended
Any screens used need to be capable of being easily replaced and capable of keeping the copepods and larvae in without causing them to stick to it.

We are feeding parvocalanus and using a 55 micron screen.


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