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Old 05/02/2016, 06:49 PM   #26
aquatictec
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New Batch New system

I have been thinking of making a new system to try. The last batch had all just died and we had another thousand plus released yesterday. I started with a 55 gallon tank and made 2 3 sided inserts to make an octagon with the water surface and tank bottom acting as 2 of the sides. All sides are 6.75 inches long
I positioned one vertical side 5 inches from one side of the 55
I made the inserts and then could not get them in the aquarium so I had to reheat them and bend them in the tank. Do not touch hot acrylic. I have a burn on my arm as a result of this. I cut out a 8 by 4 patch on the top of both sides and glued a 55 micron screen over the openings
I am calling the Kresiel the roundish area where the larvae are kept
In the water to the left of the Kreisel we have a very small pump that pumps water at a trickle to the right side of the tank.
In the right side of the tank we have 2 sponge filters a phosphate reactor and a protein skimmer
The skimmer is on a timer to go on from 11 at night to 11 in the morning so that it doesn't take phytoplankton out of the water
The tank is illuminated for 12 hours with a 36 watt LED bar
In the morning and at night we add isochrysis to tint the water and make the copepods more visible
The iso is strained through a 100 micron mesh bag.
We wait about 15 minutes and then perform a copepod density count and adjust it to about 8 copepods per ml.
circulation within the Kreisel is achieved by using 2 rigid airlines to release bubbles at the bottom of one vertical sides of the Kreisel.
A nice thing about the new system is that the larvae are much easier to see
They are capable of maintaining position in the water or moving.
Between feedings they eat about 4-5 copepods per ml

A little math:
The Kreisel area hold about 10 gallons of water
that is about 100 larvae per gallon
or 1 larvae per 38 ml
which means each larvae is eating 152 copepods per feeding 2x daily
if they do this in a 12 hour period they are consuming a copepod every 2.4 minutes

We are feeding parvocalanus copepods



Last edited by aquatictec; 05/02/2016 at 06:51 PM. Reason: forgot
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Old 05/06/2016, 05:17 PM   #27
aquatictec
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05/06/2016 update

05/06 Everything is working great. It doesn’t appear that we have lost any larvae. They seem to be bigger and darker brown. Next week we will start taking pictures to document their growth


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Old 05/07/2016, 12:42 PM   #28
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6 day old Larvae



Pictures taken May 7th 2016 in the Lab at Aquatic technology


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Old 05/07/2016, 01:04 PM   #29
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how do you sex these shrimp?


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Old 05/08/2016, 12:56 PM   #30
aquatictec
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sexing harlequin shrimp

Female harlequins are the larger of the two and have blue/purple spots under the tail.

Male harlequins are generally much smaller than the female and they lack the blue/purple spots under the tail


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Old 05/12/2016, 11:00 AM   #31
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update

There aren't that many left maybe 20
I cant attribute their deaths to anything in particular

They seem to release larvae every 13 to 18 days so they are due for a new release soon.

I am going to build 2 new systems with gasketed screens so we can easily clean them. That will allow us to flow more water through the Kreisel. I wont have all the parts I need until the 23rd so the next batch is going to go in the current system. In the meantime I am going to add a UV sterilizer.


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Old 05/23/2016, 02:01 PM   #32
aquatictec
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harlequin update

A few of the last batch made it to day 21 tying our record to date.
We had a new batch of 1000 plus born on the 19th of May. They are all going strong. as of day 4
We painted the bottom or the Kreisel white, added stick on black paper to the 2 open sides, raised the temperature to 80 and added a UV since the last batch.

We have plans to make at least 2 more systems in the next few months
The Kreisels will have removable gasketed screens, one side painted black, and we will probably try a black bottom on one. We may also experiment with lighting.


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Old 05/23/2016, 03:47 PM   #33
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This is really cool. Thanks for sharing with the ReefCentral community! Best of luck, I know raising saltwater fish and inverts is tough, but worth the effort when ultimately successful.


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Old 05/23/2016, 04:02 PM   #34
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If you have trouble with them being sucked onto the screens, make the screen area much larger, of course keeping the flow the same. That will decrease the face velocity through the screen and hopefully the larva will not be drawn onto the screen or hopefully can free themselves if they do.


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Old 05/26/2016, 03:57 PM   #35
aquatictec
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screens

The new Kreisels have 2 roughly 8" by 4" screens. They take up the whole top section on both sides.

The next system will have easily removable gasketed screens. I just obtained some 50 micron stainless steel screen. It should be a lot easier to clean.

The screens clog mostly with copepod waste. The shrimp do not get stuck in them.

We haven't had much problem with clogged screens with the new system. If a screen gets dirty the water level on the small side reservoir drops and the pump slows down. The pump pumps water from the small reservoir over the Kreisel to the large reservoir where all the equipment is. When we see the water level is down we clean the screen by rubbing it. (We will be able to remove and clean it in the new Kreisels system if we drop the water level first)

Water circulation in the Kreisel is from air bubbles
The transfer pump just moves water from one reservoir to the other, where it flows back by gravity through the Kreisel. We probably get around 10 turnovers per day of the water in the Kreisel.


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Old 05/29/2016, 03:22 PM   #36
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Today is 10dph on the latest batch
They are doing great
We are struggling on feeding them
We are using around half a million copepods per day
Our iso culture is struggling to keep up
They keep eating more and more copepods which requires more iso
We probably need to scale our production to something larger than 5 gallon buckets and carboys


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Old 06/14/2016, 02:38 PM   #37
aquatictec
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25dph update

Each day is a new record for us. Today is 26dph.
Here are some pictures taken yesterday:









The new system is working great but the screens are clogging. We are building some new systems with gasketed removable/replaceable screens and should have them on line next week.


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Old 06/20/2016, 05:48 PM   #38
aquatictec
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all gone

We made it to 30dph
We should have another batch in about a week
The new system is in place and has screens that can be swapped out
We built a stand for 8 systems but only have one set up so far.
Its expensive. We already have a blower but each system needs a stand, 55 gallon tank, 25 watt UV, a light, custom Kresisel, 2 sponge filters, protein skimmer, phosphate reactor, heater and 2 pumps. We have almost 1k in a system


We will probably take the system down we just used and sterilize it and modify it to be like the new one

The phyto cultures are all going strong
The parvo cultures are all going strong

I purchased a stainless steel 55 micron screen. It appears to work much better than the nylon screens but I wonder how safe it will be with larval shrimp


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Old 06/27/2016, 04:58 PM   #39
aquatictec
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Here we go again

The previous system has been repurposed for raising black perculas.
I moved it up front out of the clean room because I did not want rotifers to contaminate our copepod cultures.
I removed the Kreisel, tapped it for removable screens and replaced it. We should be getting a batch of eggs ready to hatch this week.
A customer who has been trying to raise them is providing the eggs to us.

We set up a new Kreisel system in the clean room
It is the same as the old one but it has removable screens and a black bottom instead of a white bottom.
The current screen is 55 micron stainless steel.

Yesterday our female harlequin shrimp released just a few larvae. This morning there were thousands. There were way more than we have ever collected. We collected them but due to the large number did not bother counting them. There are probably somewhere between 1 and 2 thousand.


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Old 07/09/2016, 01:13 PM   #40
aquatictec
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12dph update
harlequins doing fine
stainless screens suck
we received the clown eggs but only 4 hatched
they are doing fine


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Old 08/11/2016, 06:28 PM   #41
aquatictec
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We had poor success on the last 2 hatches
they only made it to around 18 days

We saved just 500 from a release of over 2000 on August 8 and 9 and have them in a Kreisel currently
Maybe we will have better luck with less competition for food


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Old 09/08/2016, 06:55 PM   #42
aquatictec
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Harlequin shrimp update 09/08/2016
still no success
We have a new system with an octagonal Kreisel built into a 55 gallon aquarium.
It holds about 8 gallons of water
It is much easier to see the larvae
The Kreisel is located about 8 inches from one side of the aquarium
at night water is pumped from one side of the aquarium to the other so the water flows through the Kreisel screens. Effectively changing the water daily.
We also only kept 500 of the last batch since food sometimes becomes an issue as they get older.
one side of the aquarium houses a uv filter, 2 sponge filters a protein skimmer and a phosphate reactor
We have removable 55 micron screens on both sides of the Kreisel
The current batch 08/29 is going strong Losses if any have been minimal


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Old 09/26/2016, 03:31 PM   #43
Nick30G
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Was just there yesterday and saw a few of those Harlequin Shrimp enjoying a nice tasty starfish.. It was the first time we have been to your place too, absolutely amazing selection I was amazed when I walked into Aquatic technology! Currently prepping for a new 30 gallon tank with 10 gallon sump and hope to get some fish from you when its ready!


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Old 09/27/2016, 06:13 PM   #44
aquatictec
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new record tomorrow

Today is 30dph
We have a lot of larvae left and they are bigger than 1/16 of an inch
Tomorrow will be a new record.


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Old 10/01/2016, 05:52 PM   #45
aquatictec
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Going strong

35dph and going strong


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Old 10/14/2016, 09:38 PM   #46
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Good luck, I am appreciate your dedication


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Old 10/19/2016, 01:01 PM   #47
aquatictec
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51dph

51dph and we still have some going but none have settled yet
fingers crossed


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Old 10/21/2016, 10:39 AM   #48
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Awesome work!


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Old 10/25/2016, 06:23 PM   #49
aquatictec
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We Did IT !!!!!

After years of trying we finally succeeded in getting them to settle
52dph we had our first 2 settlers. There was something in the tank that ate them and the rest of their brothers but we managed to save one and move it to a different system. He is about 2mm long




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Old 11/23/2016, 06:48 PM   #50
aquatictec
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status 11/23/2016

The last batch only made it to 3 days past settlement
something ate them

The current batch hatched on October 28th and are doing fine
They are half way there to settlement
In the next week or so we are going to move them to a new
identical sterile system
when/if they settle we will move them to another system with fresh asterina starfish


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