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Old 01/03/2018, 10:28 AM   #1
aby
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How do You Culture Nannochloropsis?

Can someone please tell me how to culture nannochloropsis?

I don't have any idea, but after seeing below Youtube video:

https://www.youtube.com/watch?v=SaAFQlFC7fI

I think I understand how to do it.

All I need to do is buy a 1 Liter starter package of nannochloropsis, then divide it into 2 mineral water bottle (1 Liter each) and add this 2 Liter of nannochloropsis with saltwater until full. Then I need water pump into each of this 2 Liter to provide an oxygen. How long to wait until the nannochloropsis full grown?

Am I correct?


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Old 01/03/2018, 01:08 PM   #2
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It is pretty easy, and mostly what you said. If you buy a liquid sample then yes, split the sample into two bottles, fill the bottles with the sample and new salt water. Run some airline into each from an air pump to get it bubbling, add light. Also helps to fertilize, Gullard's f/2 is the way to go.

Most any white light will do, this was my first set up, culturing both Nanno and Tetraselmis.


Upgraded to make it a little neater:


I tend to split it weekly, you should see the bottles get nice and dark green. They do sell density measuring sticks to stick in the bottles and know how dense the culture is, but I've never bothered with it.


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Old 01/03/2018, 01:15 PM   #3
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I should add, you are brand spanking new to this from your other posts. While culturing phyto is kinda easy, you really don't need it. I do it for fun, to feed some other things I culture. I'd highly suggest you do not start this, until you have your tank up and running and you understand the care going into the tank itself. Not to be too critical, but when you are posting trying to figure out what a corner overflow is called, you definitely don't have much need to be trying to learn to culture phytoplankton.


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Old 01/03/2018, 01:37 PM   #4
aby
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So, is dark green a sign of growing nannochloropsis?

Also, I know nannochloropsis is a food for copepod but what tetraselmis for?


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Old 01/03/2018, 02:23 PM   #5
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Yes if the color darkens a lot, it is growing well. And actually in my experience, nanno being non-motile does not make a good food source for my pod cultures. I would use it and the water would stay green, indicating they weren't eating it, plus there is a slightly hard shell around the nanno cells that pods struggle with. Tetraselmis is another phytoplankton, motile and with less of a outer shell, making it a better food.


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Old 01/04/2018, 11:52 AM   #6
aby
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Quote:
Originally Posted by sde1500 View Post
...Tetraselmis is another phytoplankton, motile and with less of a outer shell, making it a better food.
Better food for what?


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Old 01/04/2018, 11:55 AM   #7
sde1500
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You mentioned pods, so pods.


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Old 01/04/2018, 01:59 PM   #8
aby
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Are you sure Tetraselmis is a better food for copepods than nannochloropsis?

Why other site say and suggest nannochloropsis is most common food for copepods?


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Old 01/04/2018, 02:10 PM   #9
sde1500
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Common and better have two wildly different meanings.

Why do you want to culture phyto and pods?


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Current Tank Info: 65 gallon mixed reef, Eshopps sump and HOB overflow, RO-110int skimmer, Reefbreeder 32" photons V1.
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Old 01/04/2018, 02:15 PM   #10
aby
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Quote:
Originally Posted by sde1500 View Post
...Why do you want to culture phyto and pods?
I want to culture copepods for feeding my fish. To culture copepods, I need to culture phytoplankton too because it's a food for copepods.


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